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Figure 1 | Genetic Vaccines and Therapy

Figure 1

From: Relative persistence of AAV serotype 1 vector genomes in dystrophic muscle

Figure 1

Vector genome persistence. (A-C) Log graphs showing vector genome amounts in individual muscles of the lower sgca-/- mouse hindlimb at 4 or 12 months post administration of either rAAV2/1-tMCK-sgca (black bars) or rAAV2/1-tMCK-LacZ (grey bars). Greater persistence of vector genomes is observed in the sgca injected muscles (* indicates statistical significance [p-value ≤ 0.05], ** indicates p-value = 0.29). (A) Data for all muscles combined from the right (sgca injected) and the left (LacZ injected) hindlimbs at 4 or 12 months post injection. Muscles analyzed include: extensor digitorum longus (ED), gastrocnemius (Ga), soleus (So), tibialis anterior (TA), and quadriceps (Qu). (B) Combined (primarily) fast-twitch muscle data (ED and TA) at 4 and 12 months post injection. (C) Combined mixed/slow-twitch muscle data (Ga, So, and Qu) at 4 and 12 months post injection. (D) Bar graph depicting the differences in vector genome copy numbers in individual muscles at either 4 months (black) or 12 months (grey) post injection. Differences in expression levels between the two constructs were greater at 4 than at 12 months post administration. (E) Immunofluorescence image of a quadriceps muscle cryosection (2 months post rAAV2/1-tMCK-sgca administration) showing alpha-sarcoglycan located at the cell membrane (green) and nuclei maintained in the cell periphery (DAPI stain-blue). (F) β-galactosidase stained quadriceps muscle cryosection (2 months post rAAV2/1-tMCK-LacZ administration) showing staining in transduced myofibers (blue). (G-H) Images of extensor digitorum longus muscles (4 months post delivery of sgca or LacZ [respectively]). (I-J) Images of soleus muscles (4 months post delivery of sgca or LacZ [respectively]).

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