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Figure 2 | Genetic Vaccines and Therapy

Figure 2

From: DNA-vaccination via tattooing induces stronger humoral and cellular immune responses than intramuscular delivery supported by molecular adjuvants

Figure 2

VLP-based ELISA for detection of serum IgG antibody titers after DNA plasmid immunization. Six groups of mice (5 per group) were immunized with HPV16 L1 DNA on days 0, 14, 28 and 98 either by tattoo or intramuscularly without any adjuvant, in combination with prior application of cardiotoxin or in mixture with mouse GM-CSF DNA (ratio 1:1). For control, a group of mice was tattooed with mouse GM-CSF DNA. The blood was collected after 3 and 4 immunizations for the estimation of L1-specific antibodies. The end-point titration of sera was performed. The titers of L1-antibodies were determined using an absorption value of 0.4 as cut-off for ELISA.

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Genetic Vaccines and Therapy

ISSN: 1479-0556