Figure 1

Transduction of SH-SY5Y cells using lentiviral vectors pseudotyped with different lyssavirus glycoproteins. SH-SY5Y neuroblastoma cells were transduced using β-Gal-encoding lentiviral vectors pseudotyped with Rabies-PV, DuvSAF, LagNGA, and EBL1 glycoproteins. MOIs were adjusted based on functional titers (TU) determined on BHK-21 cells. Cells were processed for X-gal staining 3 days after treatment. At an MOI of 0.1 (white bars), 20.76 ± 1.1% of the cells were positive following transduction with the Rabies PV-pseudotyped vector, while only 11.56 ± 0.78% of the cells treated with the DuvSAF vector were positive. The LagNGA and the EBL1-pseudotyped vectors transduced 1.32 ± 0.20% and 1.25 ± 0.18% of cells, respectively. At an MOI of 1 (black bars), a similar pattern of transduction was observed. The Rabies PV and the DuvSAF vectors transduced 36.78 ± 1.05% and 13.13 ± 1.14% of the cells. The EBL1 pseudotype and the LagNGA vectors transduced 3.17 ± 0.35% and 2.79 ± 0.09% of the cells, repsectively. The transduction efficiency of Rabies PV-treated cells was the only one that increased as a result of a higher MOI (* p < 0.05).