Transduction of NSC-34 cells using lentiviral vectors pseudotyped with different lyssavirus glycoproteins. NSC-34 neuroblastoma-spinal cord hybrid cells (2 × 105 cells) were transduced using EGFP-encoding lentiviral vectors pseudotyped with Rabies-PV, DuvSAF, LagNGA or EBL1 glycoproteins. A VSV-G vector was also used for comparison this time. MOIs were adjusted based on particle titers determined using virion RNA and 5 × 103 vector particles per cell were used. Cells were processed for FACS 3 days later. The Rabies PV pseudotypes transduced 49.9% of NSC-34 cells, while the performance of all other pseudotypes was considerably lower. The VSV-G vector transduced 7.9% of the cells, followed by the EBL1 (5.00%), LagNGA (1.9%) and DuvSAF (1.0%).