In vitro uptake and retrograde transport of pseudotyped lentiviral vectors. DRG explants were plated in the central compartment of compartmentalized chambers. Left and right side compartments were filled with growth media supplemented with NGF to coax neurite growth into these compartments. The right compartments containing axon terminals were treated with equivalent concentrations of Rabies PV, DuvSAF, LagNGA, or EBL1-pseudotyped vectors (2 × 108 vector particles total) and fluorescence was analyzed 4 days later. A VSV-G pseudotyped vector was used as a negative control. For quantification of transduction, the fluorescence pixel intensity within the DRG cell bodies was quantified. Fluorescence could only be detected in DRG explants of chambers treated with the Rabies PV (57.12 ± 1.70%) and the DuvSAF (20.6 ± 5.27%) vectors, indicating their exclusive ability to be taken up and be retrogradely transported to the cell bodies of the DRG explants as opposed to the other pseudotypes. Differences between vectors were statistically significant in all groups (#, ## p < 0.05). Scale bar = 20 μm.