Figure 4
From: Anti-tumor effects of a human VEGFR-2-based DNA vaccine in mouse models
Characterization of the auto-antibodies. Western blotting(A) and ELISA(B) showed that the VEGFR-2 protein was recognized by the sera isolated from mice immunized with human VEGFR-2 LDC, but not control sera. Lane 1 = positive control. Lanes 2–5 = samples obtained prior to immunization. Lanes 6–9 = samples obtained after immunization.(2,6 = pORF-hFlk-1 LDC; 3,7 = pORF-mFlk-1 LDC; 4,8 = pORF-mcs LDC; 5,9 = GS)