Figure 6
From: Combined therapy with cyclophosphamide and DNA preparation inhibits the tumor growth in mice
Tumor growth (mean ± SEM) in mice treated with CP and human DNA, comparing the regimen shown below with the control (n = 10). Mice received CP injections (200 mg per 1 kg body weight); 30 min afterward and during the consecutive two days, mice were injected with human DNA, 1 mg i.p. and 1 mg s.c.. Mice were preimmunized with tumor AGs by s.c. injection into the dorsal back of 20 × 106 repeatedly thawed-frozen Krebs-2 tumor cells 10 days after the last DNA administration; 10 days after immunization 1 × 106 Krebs-2 tumor cells were grafted i.m. into the right hind thigh of mice. The control group was injected with saline. There were two additional control groups; one was immunized only, and the other was immunized after the CP injection.