Figure 1

Proviral configuration of MuLV SIN vector constructs incorporating the human ADA LCR. A series of eight vectors were constructed in order to test the functionality of the human ADA LCR in the context of a retroviral vector. WTPG, SPG, and SAG are control vectors. SAGL contains the LCR 3' to the ADA promoter and eGFP gene while SAGiL has an inverted LCR in the same position. SPGL examines the ability of the LCR to function with the heterologous PGK promoter. SLAG contains the LCR 5' to the ADA promoter in the forward direction while SiLAG contains the LCR inserted in the reverse orientation. WT = U3 region is wild type; S = SIN vector with deletion in 3' U3; P = PGK promoter; G = eGFP reporter gene; A = human ADA promoter; L = human ADA LCR.