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Figure 6 | Genetic Vaccines and Therapy

Figure 6

From: Retroviral vectors encoding ADA regulatory locus control region provide enhanced T-cell-specific transgene expression

Figure 6

SHGI7AL show no improved expression with the LCR in various positions and orientations. A. SHGI7AL contains intron 7 inserted 5' to the ADA promoter, while SHGI7AiL contains the LCR in the reverse orientation. SLHGA, SLHGI7A, SiLHGA and SiLHGI7A possess the LCR 5' to the polyadenylation signal in both the forward and reverse direction respectively. These vectors were created to examine if the LCR functions independently of position and orientation. B. 293A, Jurkat, and Molt-4 cells were transduced by SHGI7AL, SHGI7AiL, SLHGI7A or SiLHGI7A vectors at MOI of 0.1. The MFI of eGFP expression was evaluated using FACS analysis.

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Genetic Vaccines and Therapy

ISSN: 1479-0556