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Figure 2 | Genetic Vaccines and Therapy

Figure 2

From: The effects of DNA formulation and administration route on cancer therapeutic efficacy with xenogenic EGFR DNA vaccine in a lung cancer animal model

Figure 2

Characterization of Sec-N'-EGFR DNA vaccines. (A) Schematic diagram of the Sec-N'-EGFR expressing vectors. The N-terminal extracellular portion of the human EGFR gene was constructed to pcDNA3.1B+myc-his plasmid. Transcription is directed by cytomegalovirus (CMV) early promoter/enhancer sequences. The plasmid was named Sec-N'-EGFR (B) Expression of Sec-N'-EGFR was evaluated with transient transfection into COS-7 cells in vitro., and western blot analysis of sec-N-terminal EGFR protein. Whole cell lysates were collected from Cells transfected with Sec-N'-EGFR (lane 2), or control pcDNA3.1B+myc-his plasmid (lane 1), and analyzed with western blotting.

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