Figure 7

Tumor infiltration of CD4+ and CD8+ T cells. Tumors were excised from mice administrated with Sec-N'-EGFR, or control DNA vector by different delivery methods. Analysis of (A) CD4+ and (B) CD8+ T cells in cryosections of tumors was performed with staining with primary antibody specific for CD4+ and CD8+ cells respectively. Peroxidase-conjugated antibody was used as secondary antibody. Dark spots, peroxidase-stained cells. Similar results were obtained from two more repeated experiments (n = 3 per group).