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Figure 2 | Genetic Vaccines and Therapy

Figure 2

From: Enhancement of the expression of HCV core gene does not enhance core-specific immune response in DNA immunization: advantages of the heterologous DNA prime, protein boost immunization regimen

Figure 2

Immunocytochemical detection of HCV core proteins. Immunocytochemical detection of HCV core expression after transfection of BHK-21 cellswith pCMVcore (A1–C1), pCMVcoreKozak (A2–C2, C4), pCMVcoreIRES (A3–C3); nontransfected BHK-21 cells (A4). Immunostaining for HCV core protein using rabbit polyclonal anti-HCVcore antibody 35-7 as primary and TRITC-conjugated anti-rabbit immunoglobulin (IgG) as secondary antibody (panel A); nuclear staining by DAPI (panel B); overlay of A and B (panel C); negative control (nontransfected BHK-21 cells) after staining (A4). Fluorescent images A1–4, B1–3, C1–3 and A4 were taken with Leica DM 6000 B microscope and a Leica DFC 480 camera, and confocal image of cells transfected with pCMVcoreKozak and showing cytoplasmic, granular distribution (C4) with a Leica TCS SP2 SE.

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Genetic Vaccines and Therapy

ISSN: 1479-0556