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Figure 5 | Genetic Vaccines and Therapy

Figure 5

From: Enhancement of the expression of HCV core gene does not enhance core-specific immune response in DNA immunization: advantages of the heterologous DNA prime, protein boost immunization regimen

Figure 5

Core-specific immune response in BALB/c mice immunized with one 100 μg dose of pCMVcoreKozak (n = 7), pCMVcoreIRES (n = 6), pCMVcore (n = 6), and empty vector (n = 7). The highest titers of IgG specific to core reached throughout immunization (A); T-cell proliferation measured as the stimulation index (SI) in response to recombinant HCV cores aa 1–98 and aa 1–173 and peptide representing HCV core aa 133–142 (B); the levels of core-specific IFN-γ, IL-2, and IL-4 secretion in the cell culture fluids collected after splenocyte stimulation with HCV core aa 1–98 (C). Cytokine secretion in BALB/c mice is represented by the amounts detected in the pooled cell culture fluids from the T-cell proliferation test; therefore, no standard deviations are presented.

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Genetic Vaccines and Therapy

ISSN: 1479-0556