Reapplication at different developmental stages. EGFP expression two months after AAV-EGFP injection (filled triangles) following AAV expositions (open triangles) at different developmental stages. Animals received intrahepatic AAV-MOCS1 injections as indicated on top of the lines and an intrahepatic AAV-EGFP injection 2 months after the last AAV-MOCS1 injection. Liver sections of 2 animals are shown for each time point. Positive control animals (+) received only an AAV-EGFP injection. Negative controls (-) received no AAV. Further details are describe in figure 1.