Figure 3

Tumour protective effects of the various vaccination regimens. (n = 6) Regimen 1 - a) Time of tumour appearance - the mean time of tumour appearance was comparable in various groups (p = 0.07). b) Representative tumour growth curve - phPSA immunised mice had low tumour volumes but the difference was not significant (p vs empty vector = 0.34, vs untreated = 0.27). c) Representative Kaplan Meyer survival curve - mean survival in the immunised group was significantly prolonged (p vs empty vector = 0.04, vs untreated = 0.03). Regimen 2 - d) Time of tumour appearance - the phPSA treated mice remained tumours free for prolonged period of time (p < 0.01). e) Representative tumour growth curve - tumour growth was retarded in the immunised group. The tumour volumes were significantly lower than the untreated group at all time points (p = 0.04), but not when compared with empty vector group (p = 0.07). f) Representative Kaplan Meyer survival curve - immunisation with phPSA provided significant prolonged survival of the treated mice (p vs empty vector = 0.01, vs untreated = 0.01). Regimen 3 - g) Time of tumour appearance - mean time of tumour appearance was delayed significantly as compared to both control groups (p < 0.01). h) Representative tumour growth curve - tumour growth was significantly retarded in phPSA immunised group (p vs empty vector = 0.04, vs untreated = 0.01). i) Representative Kaplan Meyer survival curve - average survival in immunise group was significantly prolonged (p vs empty vector < 0.01, vs untreated < 0.01). Data are expressed as means ± SEM.