Figure 6

In vivo adoptive transfer of lymphocytes and antigen specific response. a) Low tumour volumes were observed in mice (n = 6) receiving splenocytes from immunised group and this slow growth of the tumours provided a prolonged survival. b). Data are expressed as means ± SEM. c) Antigen specific responses - groups of mice (n = 6) were immunised (regimen 3) and challenged s.c. (1^st tumour challenge) either with wild TRAMPC1 or transfected TRAMPC1 (TRAMPC1/hPSA). After surgical excision of the tumours and 30 days period of observation, mice were rechallenged (Tumour rechallenge), either with wild type TRAMPC1 or TRAMPC1/hPSA cells. The vaccine response was antigen specific, as on re-challenge tumour protection was only observed in mice with neo-adjuvant immunisation and rechallenge with TRAMPC1/hPSA. Only 33% mice developed tumour after rechallenge, while remaining mice remained tumour free for more than 100 days post rechallenge with TRAMPC1/hPSA.