Figure 1

Western blot analysis of Vero cells transfected with (a), H5 and (b) MDP1 genes. Expression of H5 protein (product of ~ 64 kDa) was detected 72 hours after transfection while the expression of MDP1 protein (product of ~ 31 kDa) was detected 48 and 72 hours after transfection. (A) Lane M is Prestained™ protein marker (Invitrogen^®, USA); Lane 1, 2 and 3 are Vero cells harvested 72, 48 and 24 hours, respectively, after transfection of pcDNA3.1 + with H5; Lane 4 is the non-transfected Vero cells. (B) Lane M is Prestained™ protein marker (Invitrogen^®, USA); Lane 1, 2 and 3 are Vero cells harvested 72, 48 and 24 hours, respectively, after transfection of pcDNA3.1 + with MDP1; Lane 4 is the non-transfected Vero cells.