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Table 2 IFNγ ELISPOT data for the ialB and omp25 based vaccines showing the contribution of CD4+ and CD8+ T cells to the total response.

From: Liposomal delivery of p-ialB and p-omp25 DNA vaccines improves immunogenicity but fails to provide full protection against B. melitensischallenge

Vaccine

Net responsive cells

 

Total cell population

CD4+ depleted cell population

CD8+ depleted cell population

Stimulation with Omp25 (10 μg/ml)

p-omp25 [X1]

50.8 ± 24.9

27.8 ± 2.3 [↓45%]

11.3 ± 2.3 [↓78%]

p-omp25 [X4]

73.3 ± 20.3

41.0 ± 5.2 [↓44%]

10.8 ± 3.5 [↓85%]

L-p-omp25

25.0 ± 0.77

2.8 ± 0.08 [↓89%]

13.0 ± 0.9 [↓48%]

Stimulation with IalB [15 μg/ml]

p-ialB [X1]

12.75 ± 1.75

0

0

p-ialB [X4]

75.02 ± 18.37

11.0 ± 4.85 [↓85%]

36.25 ± 2.92 [↓52%]

L-p-ialB

23.25 ± 6.06

0

0