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Figure 4 | Genetic Vaccines and Therapy

Figure 4

From: Combined vascular endothelial growth factor-A and fibroblast growth factor 4 gene transfer improves wound healing in diabetic mice

Figure 4

Skin morphology of db/db mice 21 days after wounding and gene transfer. (A) Haematoxylin/eosin staining of the skin injected with (I) PBS; (II) AAV-LacZ; (III) AAV-FGF4-IRES-GFP; (IV) AAV-VEGF-A and (V) AAV-FGF4-IRES-VEGF-A. Analysis revealed less adipose tissue and better organized granulation tissue with the presence of hair and restoration of normal architecture of dermis in AAV-VEGF-A and AAV-FGF4-IRES-VEGF-A-treated mice in comparison to PBS-, AAV-LacZ- and AAV-FGF4-IRES-GFP-injected animals. Panels are representative of 5 animals per group. Scale bar (I-V) = 0.1 mm. (VI) Higher magnification of AAV-FGF4-IRES-VEGF-A-injected skin with granulomas (arrows). Scale bar (VI) = 0.05 mm. (B) Representative Masson's trichrome staining of the skin injected with (I) PBS; (II) AAV-LacZ; (III) AAV-FGF4-IRES-GFP; (IV) AAV-VEGF-A and (V) AAV-FGF4-IRES-VEGF-A. Double-headed arrows indicate the thickness of the collagen layer that was significantly thicker after injection of all three therapeutic vectors (AAV-FGF4-IRES-GFP, AAV-VEGF-A, AAV-FGF4-IRES-VEGF-A) when compared to PBS- and AAV-LacZ-treated animals. Panels are representative of 5 animals/group. Scale bar = 0.05 mm.

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Genetic Vaccines and Therapy

ISSN: 1479-0556