Figure 2

Effects of the Igk MAR in lymphoblastoma and neural progenitor cells. Raji (a), Jurkat (b) and neural progenitor (c) cells were transduced using SIN integrating (IN WT) or non-integrating (IN N) lentiviral vectors expressing the luciferase transgene with (black columns) or without (white columns) the Igκ MAR. Raji and Jurkat cells were seeded in 24-well plates at a density of 100,000 cells per well. Neural progenitor cells were seeded in 96-well plates at a density of 10,000 cells per well in a N2 standard medium supplemented with 10 ng/ml bFGF. After contact with the vectors, neural progenitors were maintained undifferentiated or were glially differentiated by addition of 10% fetal calf serum in the culture medium. Unpaired t test was performed to analyze results of figures a and b and two-way ANOVA for figure c. Statistical significance of the t test (figure b, * for p < 0.05) or the Bonferroni post-test (figure c, *** for p < 0.001) are represented on the relevant columns.