Table 1 Primers used for cloning to amplify promoters with underlined restriction sites.
From: Comparative analysis of macrophage associated vectors for use in genetic vaccine
Promoter (Constructs) | Primer Sequence (5' → 3') | Restriction site |
|---|---|---|
Macrosialin (pAcGFP-MS) | F-TATTAATGACCAAATCTACAGGGAGAACCC | VspI/Eco47III |
| Â | R-AGCGCTAGATGCTCAGACCAGCTA | Â |
EMR-1 (pAcGFP-EMR) | F-TCATATGGAATTCTTTGTTTAGGTCTGTATGC | NdeI/Eco47III |
| Â | R-TAGCGCTTACTGTGGCAGTCATTCA | Â |
Beta-5 Integrin (pAcGFP-B5I) | F-CCGATTAATATTCAAACGCCTTAGGTAGGTTT | VspI/Eco47III |
| Â | R-AGCGCTTCTACTCTCGGAGACCCT | Â |