Table 2 Nucleotide sequence of primers and probe used in pGEM-T Easy cloning and qRT-PCR assay.
From: Comparative analysis of macrophage associated vectors for use in genetic vaccine
Assay | Primer/Probe | Sequence (5'→ 3') | Nucleotide positions |
|---|---|---|---|
qRT-PCR | Forward Primer | TACGGCGTGCAGTGCTTCT | 196-214 |
| Â | Reverse Primer | AGATGGTGCGCTCCTGGAT | 277-295 |
| Â | TaqMan Probe | CTACCCCGATCACATGAAGCAGCACG | 219-244 |
Cloning | Forward Primer | AAGTTCATCTGCACCAC | 133-149 |
| Â | Reverse Primer | TGTAGTTGCCGTCATCCT | 302-319 |