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Figure 1 | Genetic Vaccines and Therapy

Figure 1

From: Establishment of stable Huh-7 cell lines expressing various hepatitis C virus genotype 3a protein: an in-vitro testing system for novel anti-HCV drugs

Figure 1

a) Showing the amplified genes of Core (573), Envelope 1(576), Non-structural 2 (NS2, 642 bp) and Non Structural 4a (NS4A, 168 bp), b) lanes 2-5 (left to right) showing the complete amplified region of 783 bp of Non Structural 4b gene. From the HCV positive serum with 3a genotype, RNA was extracted and individual gene was reverse transcribed using M-MLV. HCV reference sequence of NZL1 # D17763 was used for primer designing on Primer 3 software, restriction sites and kozak sequences were added after restriction analysis on web cutter and neb-cutter primers sequences. Each entire gene was amplified individually. Amplified genes with restriction sites were then cloned in mammalian expression vector PcDNA3.1+.

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Genetic Vaccines and Therapy

ISSN: 1479-0556