Figure 3
From: Biodistribution and blood clearance of plasmid DNA administered in arginine peptide complexes
Effects of DNA dose on plasmid DNA expression after delivery in arginine/DNA complexes. Various amounts of plasmid DNA complexed with arginine peptide at an N/P ratio of 3:1 were intraperitoneally administered to mice, and mRNA levels were evaluated using real time RT-PCR. Total RNA was extracted from the organs. After preparation of cDNA, PCR amplification of luciferase and GAPDH genes was performed using the specific primers described in the Materials and Methods section. Results are expressed as means ± S.D. for at least 3 different experiments.