Figure 4

Duration of plasmid DNA expression. Plasmid DNA (100 μg) complexed with arginine peptide at an N/P ratio of 3:1 was intraperitoneally administered to mice, and total RNA was extracted from the organs at the indicated time points. The RNA extracts were transformed to cDNA using RT-PCR to serve as templates for nested PCR analysis. PCR amplification of luciferase and GAPDH genes was performed using the specific primers described in the Materials and Methods section. The nested PCR products were separated on a 1.2% agarose gel.