Figure 2From: HCV entry receptors as potential targets for siRNA-based inhibition of HCVSilencing of gene expression of HCV receptors by their specific siRNA in a dose dependent manner. Huh-7 cells were infected with HCV-3a serum (S3a) along with or without 25 nM, 50 nM and 100 nM of siRNAs against HCV receptors CD81, LDLR, CLDN1 and SR-BI and scrambled (Sc) siRNA for 48 hrs. The scrambled (Sc) siRNA has nonspecific sequence with no homology to any known cellular genes. A) Semi-quantitative PCR analysis of gene expression of CD81, LDLR, CLDN1 and SR-BI using serial doses (25 nM, 50 nM and 100 nM) of scrambled siRNA or the specific siRNA siCD81, siCLDN, siLDLR, or siSR-BI respectively. B) Real-time PCR analysis indicating fold reduction of CD81, CLDN1, LDLR, and SR-BI gene using serial doses (25 nM, 50 nM and 100 nM) of scrambled siRNA or specific siRNAs of siCD81, siCLDN, siLDLR or, siSR-BI respectively.Back to article page