Figure 4
From: HCV entry receptors as potential targets for siRNA-based inhibition of HCV
Combined effect of specific siRNA silencing of HCV receptors on viral titer in Huh-7 cells. Huh-7 cells were treated with scrambled siRNAs (Sc) or combinations of siRNAs (siCD81-B+siLDLR, siCD81+siCLDN1, siCD81+siSR-BI, siLDLR+ siCLDN1, siLDLR+ siSR-BI, siCLDN1+siSR-BI) and incubated for 6 hrs before adding HCV-3a sera (Ser 3a). HCV RNA levels were quantified by real-time PCR. Data are expressed as mean percent viral load of non-siRNA treated samples. Three independent experiments with triplicate determinations were performed. Error bars indicate indicate means plus or minus SD. *p < 0.01vs. Ser3a.