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Figure 3 | Genetic Vaccines and Therapy

Figure 3

From: In vitro evaluation of a double-stranded self-complementary adeno-associated virus type2 vector in bone marrow stromal cells for bone healing

Figure 3

BMP-2 Production by Viral Transduced Cells 1 million rat bone marrow stromal cells (BMSCs) and 293T cells were transduced with AAV-BMP-2, LV-BMP-2 and Ad-BMP-2 and the BMP-2 production was quantified 48 hours after transduction. Non-transduced cells served as negative control. A) Amongst the three viral vectors used to over express BMP-2, AAV-BMP-2 induced the highest amount of BMP-2 production in 293T cells. Transducing the 293T cells with Ad-BMP-2 induced cell death after 24 hours in culture. B) AAV-BMP-2 transduced rat bone marrow stromal cells (BMSCs) did not produce any detectable amount of BMP-2 as opposed to LV-BMP-2 and Ad-BMP-2 transduced rat bone marrow stromal cells which made significantly higher amounts of BMP-2.*: P value < 0.05 compared to all other groups, †: P value < 0.05 compared to non-transduced group

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