Phenotypical analysis of memory cells in the spleen of reconstituted BKO mice. 1 × 106 electroporated B lymphocytes (pcDNA3-Hsp65 or mock vector) were injected by endovenous route (orbital plexus) in WT and B cell deficient mice. After seven days, the mice were euthanized for analysis of CD44 and CD62L on CD8 (A) and CD4 (B) T cells, by flow cytometry. Assays were performed in triplicate and the results represent the mean ± SD of at least two independent experiments. *P < 0,05 versus wild-type mice (One-way ANOVA with Tukey's post-test).