Double suicide genes driven by kinase domain insert containing receptor promoter selectively kill human lung cancer cells

Table 1 Cell survival rate in HepG2, L9981 and NL9980 cells with or without the double suicide gene system.

Cell line	Group	Cell survival rate (%)				p
		Control	GCV(5 ug/ml)	5-Fc(100 ug/ml)	GCV(5 ug/ml)+ 5-Fc(100 ug/ml)
HepG2	HepG2	97 ± 5	96 ± 3	99 ± 1	95 ± 2	0.16
	HepG2-Vector	100 ± 3	97 ± 3	98 ± 1	99 ± 3	0.38
	HepG2-CDglyTK	99 ± 3	97 ± 6	94 ± 5	96 ± 3	0.57
	p	0.201	0.17	0.13	0.20
L9981	L9981	96 ± 3	94 ± 2	95 ± 6	94 ± 4	0.08
	L9981-Vector	94 ± 3	95 ± 3	97 ± 5	95 ± 3	0.09
	L9981-CDglyTK	94 ± 5	67 ± 2	71 ± 4	34 ± 3	0.01
	P	0.32	0.002	0.0025	0.0021
NL9980	NL9980	96 ± 1	96 ± 3	95 ± 2	97 ± 4	0.1
	NL9980-Vector	93 ± 3	94 ± 2	95 ± 4	95 ± 3	0.11
	NL9980-CDglyTK	96 ± 5	63 ± 1	66 ± 5	36 ± 4	0.00
	P	0.22	0.03	0.011	0.00

The MTT method was used to determine cellular viability. 5-Fc and/or GCV treatment significantly decreased the cellular survival rate in L9981 cells with CD and TK transgenes. The cells treated with 5-Fc and GCV together showed the maximal reduction.