Skip to main content

Table 2 Cell apoptosis index in HepG2, L9981 and NL9980 cells with or wtihout the double suicide gene system.

From: Double suicide genes driven by kinase domain insert containing receptor promoter selectively kill human lung cancer cells

Group

Apoptosis index(AI %)

P

 

HepG2

HepG2-vector

HepG2-CDglyTK

 

0.9% N S

6.3 ± 1.0

5.7 ± 0.3

6.3 ± 1.3

0.43

GCV(5 μg/ml)

7.1 ± 0.6

6.6 ± 0.7

7.2 ± 0.7

0.51

5-FC(100 μg/ml)

6.8 ± 0.7

7.0 ± 1.0

7.0 ± 0.9

0.16

GCV(5 μg/ml)+5-FC(100 μg/ml)

5.9 ± 0.5

6.1 ± 1.1

6.6 ± 1.1

0.18

P

0.07

0.12

0.34

 
 

L9981

L9981-vector

L9981-CDglyTK

 

0.9% N S

5.1 ± 1.1

5.7 ± 1.3

5.4 ± 0.7

0.07

GCV(5 μg/ml)

5.5 ± 0.3

6.0 ± 0.3

9.7 ± 1.7

0.04

5-FC(100 μg/ml)

6.0 ± 0.8

6.7 ± 1.3

13.1 ± 2.7

0.031

GCV(5 μg/ml)+5-FC(100 μg/ml)

6.1 ± 0.2

6.1 ± 1.1

19.9 ± 4.2

0.026

P

0.19

0.17

0.012

 
 

NL9980

NL9980-vector

NL9980-CDglyTK

p

0.9% N S

6.0 ± 0.1

5.9 ± 0.3

5.8 ± 1.7

0.12

GCV(5 μg/ml)

6.5 ± 0.2

6.3 ± 1.3

11.2 ± 2.4

0.03

5-FC(100 μg/ml)

6.1 ± 0.8

5.7 ± 0.8

12.9 ± 3.6

0.024

GCV(5 μg/ml)+5-FC(100 μg/ml)

5.9 ± 0.3

6.1 ± 1.5

23.1 ± 5.0

0.021

P

0.11

0.11

0.0092

 
  1. Cells were stained with popidium iodide and analyzed with flow cytometry to detect apoptosis. 5-Fc and/or GCV treatment did not change the cellular apoptosis in the HepG2 cells with or without CD and TK transgenes.