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Figure 3 | Genetic Vaccines and Therapy

Figure 3

From: In-vitro model systems to study Hepatitis C Virus

Figure 3

Scheme of HCVpp production and infection. HEK-293T cells were transfected with CMV-Gag-Pol, Luciferase vector and HCV GP expression constructs. Successfully transfected 293T cells assemble HCVpp intracellularly and secrete them into the supernatant. The HCVpp-containing supernatant can be harvested. The supernatant can then either be used unmodified or conditioned (centrifugation, drug treatement etc.) to infect target cells. HCVpp attach to the target cells, become endocytosed and fuse with the endocytic membranes to release the retroviral core containing the luciferase vector into the cytoplasm. The Luciferase vector is then reverse transcribed and integrated into the host-cell genome. 24 to 96 hours after infection, transgene expression can be analyzed.

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