A comparison of multiple shRNA expression methods for combinatorial RNAi

Mcintyre, Glen J; Arndt, Allison J; Gillespie, Kirsten M; Mak, Wendy M; Fanning, Gregory C

doi:10.1186/1479-0556-9-9

Genetic Vaccines and Therapy

Table 2 Multiple domain, single transcript sequences

From: A comparison of multiple shRNA expression methods for combinatorial RNAi

Comb.	Sequence ^a	Len.
R_V_T ₁	GGAACUUAAGAGUGAAGCUGUUAGACAUUACUCGAGAAAUGUCUAACAGCUUCACUCUUAAGUUCCGUAUAUUUCAAGGAAAGCUAAGGACUGGUUACUCGAGAAACCAGUCCUUAGCUUUCCUUGAAAUAUAGAAACUGCUUGUACCAAUUGCUAUUGUAAAACUCGAGAUUUACAAUAGCAAUUGGUACAAGCAGUUUGUx	204
R_V_T ₈	GGAACUUAAGAGUGAAGCUGUUAGACAUUACUCGAGAAAUGUCUAACAGCUUCACUCUUAAGUUCCGCUGCAGGUAUAUUUCAAGGAAAGCUAAGGACUGGUUACUCGAGAAACCAGUCCUUAGCUUUCCUUGAAAUAUAGGACGUCGAAACUGCUUGUACCAAUUGCUAUUGUAAAACUCGAGAUUUACAAUAGCAAUUGGUACAAGCAGUUUGUx	218
V-T	GUAUAUUUCAAGGAAAGCUAAGGACUGGUUGCUGCAGGAAACUGCUUGUACCAAUUGCUAUUGUAAAACUCGAGAUUUACAAUAGCAAUUGGUACAAGCAGUUUGGACGUCGAACCAGUCCUUAGCUUUCCUUGAAAUAUAUx	144
O'-O"	AAGACAGUCCAACACACGCCACCUGUCUCGCUGCAGGAACAGUCUGUCAAA GGUGACCCCUGUCUCACUCGAGAGAGACAGGGGUCACCUUUGACAGACUGUUGGACGUCGGAGACAGGUGGCGUGUGUUGGACUGUCUUGUx	144
T-V	GAAACUGCUUGUACCAAUUGCUAUUGUAAAGCUGCAGGUAUAUUUCAAGGAAAGCUAAGGACUGGUUACUCGAGAAACCAGUCCUUAGCUUUCCUUGAAAUAUAGGACGUCGUUUACAAUAGCAAUUGGUACAAGCAGUUUGUx	145
U-R	GGCAAUGAGAGUGAAGGAGAAGUAUCAGCAGCUGCAGGGGAACUUAAGAGUGAAGCUGUUAGACAUUACUCGAGAAAUGUCUAACAGCUUCACUCUUAAGUUCCGGACGUCGUGCUGAUACUUCUCCUUCACUCUCAUUGCGUx	145
R-U	GGGAACUUAAGAGUGAAGCUGUUAGACAUUGCUGCAGGGCAAUGAGAGUGAAGGAGAAGUAUCAGCAACUCGAGAUGCUGAUACUUCUCCUUCACUCUCAUUGCGGACGUCGAAUGUCUAACAGCUUCACUCUUAAGUUCCGUx	145
R-V-T	GGAACUUAAGAGUGAAGCUGUUAGACAUUGCUGCAGGUAUAUUUCAAGGAAAGCUAAGGACUGGUUGCUGCAGGAAACUGCUUGUACCAAUUGCUAUUGUAAAACUCGAGAUUUACAAUAGCAAUUGGUACAAGCAGUUUGGACGUCGAACCAGUCCUUAGCUUUCCUUGAAAUAUAGGACGUCGAAUGUCUAACAGCUUCACUCUUAAGUUCCUx	217
T-V-R	AAACUGCUUGUACCAAUUGCUAUUGUAAAGCUGCAGGUAUAUUUCAAGGAAAGCUAAGGACUGGUUGCUGCAGGGGAACUUAAGAGUGAAGCUGUUAGACAUUACUCGAGAAAUGUCUAACAGCUUCACUCUUAAGUUCCGGACGUCGAACCAGUCCUUAGCUUUCCUUGAAAUAUAGGACGUCGUUUACAAUAGCAAUUGGUACAAGCAGUUUUx	217
V-T ₀	GUAUAUUUCAAGGAAAGCUAAGGACUGGUUAAACUGCUUGUACCAAUUGCUAUUGUAAAACUCGAGAUUUACAAUAGCAAUUGGUACAAGCAGUUUAACCAGUCCUUAGCUUUCCUUGAAAUAUAUx	128
V ¹⁹ _- T ¹⁹	GGGAAAGCUAAGGACUGGUUGCUGCAGGCUGCUUGUACCAAUUGCUAUACUCGAGAAUAGCAAUUGGUACAAGCAGGGACGUCGAACCAGUCCUUAGCUUUCCUx	106

^a : sequences external to the target-matched dsRNA domains are shown in bold (e.g. loops, spacers, U_x termination remnants).

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