Figure 3

CIN alters production of cytokines and IgE. On day 23 of the prophylactic procedure (see Fig. 2A) spleens were removed and single-cell suspensions of splenocytes were prepared. Cells were cultured for 48 h with OVA, and the levels of secreted IFN-γ and IL-5 (A) and IL-4 (B) were measured. Total serum IgE was measured on day 23 (C). Values are means ± SEM (*p < 0.05, **p < 0.01).