PCR analysis of transduced MSCs and treated mice.
Genomic DNA isolated from MSCs from KO mice (A), brains from untreated KO mice (B), brains from wild-type mice (C), and brains from KO mice treated with MLV-IDUA-transduced MSCs (D) was used to analyze the IDUA and β-actin genes. The three molecular markers in the figures represent 300, 200 and 100 bp. N = PCR negative control; P = PCR positive control; I = IDUA; En = Endogenous β-actin; NT = non-transduced cells. The numbers on the figures indicate the number of each animal.