Figure 2

Immune responses induced by different means of id DNA vaccine delivery. 10 μg pKCMVp37B was diluted in 10 or 100 μl saline and delivered by needle or Biojector (BJ), respectively. Also mice immunized with needle or Biojector followed by electroporation (EP) were included. Immunizations were done week 0 and 4. A) Cellular immune responses were assessed with IFN-γ ELISpot on splenocytes collected two weeks after the second immunization. A peptide pool representing Gag p24B was used to stimulate splenocytes. B) Gag-specific IgG titers in serum collected at the same time point were determined by Gagp17/p24B ELISA. Bars represent mean values. *Significant difference (p < 0.05) and **significant difference (p < 0.01). ns = no significant difference.