Table 1 Summary of the results obtained from cells transfected with chloramphenicol acetyl transferase using the seven different transfection reagents tested. Results are given as a range across all cell types.
Liposome | Manufacturer | DNA amount | Liposome: DNA ratio | Transfection Efficiency | % Cell Survival |
|---|---|---|---|---|---|
FuGENE 6 | Roche | 1 and 2 μg | 3:1 | 3.0 – 16.4 | 65 – 80 |
Lipofectamine 2000 | Invitrogen | 2 and 4 μg | 3:1 | 0.4 – 34.0 | 9 – 27 |
Lipofectamine PLUS | Invitrogen | 0.8 and 1.6 μg | 3:1 | 3.4 – 18.3 | 18 – 61 |
Metafectene | Biontex | 2 and 4 μg | 3:1 | 0.6 – 8.6 | 25 – 50 |
Lipofectin | Invitrogen | 1 and 2 μg | 4:1 | 0.0 – 7.1 | 65 – 100 |
GenePORTER | Gene Therapy Solutions | 3 and 6 μg | 5:1 | 1.6 – 21.9 | 24 – 55 |
LipoGen | Invivo | 1 and 2 μg | 3:1 | 0.0 – 13.9 | 10 – 90 |