Table 1 Comparison of nucleofection parameters in ALL patient samples. Nucleofection using an RFP expression vector was carried out using 6 different patient samples (patients 4,13,8,11,19,12) from one of two potential tissue sources, PB, peripheral blood, or BM, bone marrow. 1No NF, not nucleofected autofluorescence control. 2Nucleofection was carried out with one of three solutions R, T, or V, and the following electrical settings on the Amaxa nucleofection device: T20, U15, T17, T27, S04, O17, T16, T01, O17. 3The table lists the percentage of viable cells expressing the plasmid-encoded RFP at 24 hours post-nucleofection.
Electroporation Parameters | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|
Patient Samples | No NF1 | R2/T20 | T/U15 | R/T17 | R/T27 | T/S04 | T/O17 | R/T16 | V/T01 | V/O17 |
4 PB | 30.1 | 62.3 | 61.5 | 56.8 | 52.9 | 52.5 | 46.2 | 46.1 | 40.5 | 20.9 |
13 BM | 1.0 | 45.0 | 45.6 | 42.9 | 40.2 | 32.4 | 36.1 | 38.5 | 21.3 | 36.1 |
8 PB | 1.0 | 9.2 | 10.9 | 9.3 | 6.7 | 8.2 | 5.6 | 6.4 | 6.1 | 3.7 |
11 BM | 1.2 | 17.1 | 25.2 | 13.2 | 13.4 | 9.5 | 5.7 | 10.0 | 5.7 | 7.1 |
10 BM | 0.1 | 8.0 | 10.0 | 4.6 | 4.5 | 2.3 | 2.0 | 3.9 | 2.0 | 0.9 |
12 BM | 1.3 | 2.9 | 3.6 | 2.4 | 2.3 | 2.6 | 3.5 | 1.8 | 2.1 | 3.6 |