Table 1 Summary of mRNA libraries and clone analysis. In the case of MEL14, total RNA was extracted from ~5 × 104 pleural tumor cells (NA: Not applicable)
Patients | Weight of tumor sample (mg) | Quantity of extracted total RNA (μg) | Number of clones (cfu) | Size range of analyzed clones (nt) | Quantity of mRNA library prepared (mg) | Number of injection performed | |
|---|---|---|---|---|---|---|---|
1 | MEL01 | 32.4 | 9.4 | 1 × 105 | 500 – 4000 | 2.8 | 10 |
2 | MEL02 | 33 | 10.5 | 1 × 105 | 200 – 8000 | 5.0 | 12 |
3 | MEL03 | 33.6 | 26.3 | 5 × 105 | 250 – 1000 | 1.9 | 6 |
4 | MEL04 | 38 | 36.7 | 2 × 105 | 400 – 3500 | 3.6 | 8 |
5 | MEL05 | 85 | 14.2 | 2 × 105 | 500 – 1000 | 5.0 | 13 |
6 | MEL06 | 60 | 115.2 | 3 × 105 | 300 – 1200 | 4.0 | 16 |
7 | MEL07 | 76 | 70.5 | 5 × 105 | 500 – 1200 | 2.8 | 7 |
8 | MEL08 | 34.1 | 60.9 | 3 × 105 | 500 – 1200 | 5.3 | 16 |
9 | MEL09 | 95 | 84 | 4 × 104 | 350 – 800 | 4.5 | 10 |
10 | MEL10 | 78.7 | 62.5 | 2 × 105 | 600 – 1200 | 4.2 | 16 |
11 | MEL11 | 77.3 | 9.56 | 3 × 105 | 400 – 1000 | 2.7 | 3 |
12 | MEL12 | 34.3 | 15.4 | 6 × 104 | 400 – 1200 | 3.9 | 10 |
13 | MEL13 | 72 | 9.14 | 2 × 105 | 750 – 2000 | 4.4 | 16 |
14 | MEL14 | NA | 13.2 | 1 × 105 | 400 – 10000 | 1.8 | 4 |
15 | MEL15 | 60 | 41.5 | 3 × 105 | 500 – 4000 | 4.1 | 8 |
Average | 57.8 | 38.6 | 2 × 105 | 450 – 3250 | 3.7 | 10 | |