Table 2 Detection of lacZ- phage
From: A trial of somatic gene targeting in vivo with an adenovirus vector
Packaging exp. | RAd | Genotype | Animal number | Packaging | Total number of plaque formers | lacZ- plaques | Mutant Frequency | Expected genotype |
|---|---|---|---|---|---|---|---|---|
1 | None | Not relevant | #0 | Tube 1 | 4.5 × 104 | 4 | 8.9 × 10-5 | n.t. |
|  |  |  |  | Tube 2 | 3.2 × 104 | 3 | 9.4 × 10-5 | n.t. |
|  |  |  |  | Tube 3 | 8.5 × 104 | 8 | 9.4 × 10-5 | n.t. |
|  |  |  |  |  |  |  | average 9.2 × 10-5 |  |
|  | AdNY56 | lacZ + | #1 | Tube 4 | 8.5 × 104 | 8 | 9.4 × 10-5 | n.t. |
|  |  |  |  | Tube 5 | 6.4 × 104 | 3 | 4.7 × 10-5 | n.t. |
|  |  |  |  | Tube 6 | 8.8 × 104 | 1 | 1.1 × 10-5 | n.t. |
|  |  |  |  |  |  |  | average 5.1 × 10-5 |  |
2 | None | Not relevant | #0 | Tube 7 | 4.9 × 104 | 5 | 10 × 10-5 | n.t. |
|  |  |  |  | Tube 8 | 6.6 × 104 | 4 | 6.1 × 10-5 | n.t. |
|  |  |  |  | Tube 9 | 5.1 × 104 | 10 | 20 × 10-5 | n.t. |
|  |  |  |  |  |  |  | average 12 × 10-5 |  |
|  | AdNY57 | lacZ- (Glu461Gly) | #2 | Tube 10 | 3.8 × 104 | 6 | 16 × 10-5 | 0/6 |
|  |  |  |  | Tube 11 | 3.0 × 104 | 7 | 23 × 10-5 | 0/7 |
|  |  |  |  | Tube 12 | 4.5 × 104 | 9 | 20 × 10-5 | 0/9 |
|  |  |  |  |  |  |  | average 20 × 10-5 | total 0/22 |
3 | None | Not relevant | #0 | Tube 13 | 3.7 × 104 | 6 | 16 × 10-5 | n.t. |
|  |  |  |  | Tube 14 | 6.0 × 104 | 5 | 8.3 × 10-5 | n.t. |
|  |  |  |  | Tube 15 | 4.4 × 104 | 4 | 9.1 × 10-5 | n.t. |
|  |  |  |  |  |  |  | average 11 × 10-5 |  |
|  | AdNY57 | lacZ- (Glu461Gly) | #2 | Tube 16 | 1.3 × 104 | 9 | 69 × 10-5 | 0/9 |
|  |  |  |  | Tube 17 | 3.9 × 104 | 19 | 49 × 10-5 | 0/19 |
|  |  |  |  | Tube 18 | 6.5 × 104 | 26 | 40 × 10-5 | 0/26 |
|  |  |  |  |  |  |  | average 53 × 10-5 | total 0/54 |
4 | None | Not relevant | #0 | Tube 19 | 2.6 × 105 | 8 | 8.5 × 10-5 | n.t. |
|  | AdNY57 | lacZ- (Glu461Gly) | #3 | Tube 20 | 1.6 × 105 | 5 | 6.3 × 10-5 | 0/5 |
|  |  |  |  | Tube 21 | 4.1 × 105 | 9 | 8.6 × 10-5 | 0/9 |
|  |  |  |  |  |  |  | average 1.5 × 10-5 | total 0/14 |
5 | None | Not relevant | #0 | Tube 22 | 3.3 × 104 | 3 | 9.1 × 10-5 | n.t. |
|  | AdNY58 | lacZ- (Tyr105 Stop) | #4 | Tube 23 | 8.6 × 104 | 4 | 4.7 × 10-5 | 0/4 |
|  |  |  |  | Tube 24 | 3.1 × 104 | 3 | 9.7 × 10-5 | 0/3 |
|  |  |  |  |  |  |  | average 7.2 × 10-5 | total 0/7 |