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Figure 3 | Genetic Vaccines and Therapy

Figure 3

From: Tissue distribution of a plasmid DNA encoding Hsp65 gene is dependent on the dose administered through intramuscular delivery

Figure 3

Persistence of DNA adenine methylase site methylations (dam) of pcDNA3-HSP65 in muscle at 6 months after immunization. (A) Approximately 1 μg cellular DNA obtained from muscle of immunized mouse were digested with Nde I and Dpn I (lane a), Nde I and Mbo I (lane b), or with Nde I alone (lane c) and amplified by PCR using Hsp65 primers. The samples were submitted to electrophoresis on a 1% agarose gel (B) The positive control was done using E.coli DNA digested with Dpn I, Mbo I or non-digested to show the dam methylation pattern. The mobility of DNA size standards (l DNA cut with Hind III) are shown on the left.

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Genetic Vaccines and Therapy

ISSN: 1479-0556