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Figure 4 | Genetic Vaccines and Therapy

Figure 4

From: Tissue distribution of a plasmid DNA encoding Hsp65 gene is dependent on the dose administered through intramuscular delivery

Figure 4

Analysis of the pcDNA3-Hsp65 genome integration. Samples of liver tissue from mice immunized with 100 μg of pcDNA3-HSP65 (lanes a and b) and from nonimmunized mice (lanes c and d) (negative control) were submitted to Southern blot after Nde I digestion. Lanes e and f correspond to wild-type plasmid digested with Nde I or undigested, respectively. The bands were detected using pcDNA3 labeled with chemiluminescent reagent. The multiple forms of plasmid DNA are indicated in the figure. The samples were loaded in a same gel and the lanes not used were removed.

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Genetic Vaccines and Therapy

ISSN: 1479-0556