Figure 2

Exon skipping in cultured mdx cells after transfection with AOs directed at targeted exons. Primer set 2 inner (Table 1) was used for nested PCR amplification to generate the full length product of 1357 bp (indicated). Lane numbers correspond to targeted exons. The induced exon skipping products are 1263 bp (Δ exon 19), 1115 bp (Δ exon 20), 1176 bp (Δ exon 21), 1211 bp (Δ exon 22), 1144 bp (Δ exon 23 A06), 1243 bp (Δ exon 24), 1201 bp (Δ exon 25), (Δ exon19), 1144 bp (Δ exon 23 AO10, Table 2). The 998 bp product corresponds to the removal of exons 22 and 23, a common product of exon 23 targeting.