Figure 4

RT-PCR of shortened transcripts induced in the presence of background alternative splicing. RT-PCR pattern of exon 19–25 skipping induced in an immortalized culture where there was (A) no evidence of revertant transcripts in the untreated samples, (B) low levels of endogenous alternative splicing visible in the untreated cells. Primer set 2 inner (Table 1) was used for amplification.