Table 1 Primer sequences used for nested PCR analysis
From: Induction of revertant fibres in the mdx mouse using antisense oligonucleotides
Primer set No. | Â | PCR Primer | Sequence (5'-3') | Full length product (bp) |
|---|---|---|---|---|
1 | Outer | Exon:13F Exon:27R | GCT TCA AGA AGA TCT AGA ACA GGA GC CTA TTT ACA GTA TCA GTA AGG | Â |
| Â | Inner | Exon 18F Exon 26R | GAA GCT GTA TTA CAG AGT TCT G CCT GCC TTT AAG GCT TCC TT | 1250 bp |
2 | Outer | Exon:13F Exon:27R | GCT TCA AGA AGA TCT AGA ACA GGA GC CTA TTT ACA GTA TCA GTA AGG | Â |
| Â | Inner | Exon 18F Exon 26 R | GAT ATA ACT GAA CTT CAC AG TTC TTC AGC TTG TGT CAT CC | 1357 bp |
3 | Outer | Exon:13F Exon:35R | GCT TCA AGA AGA TCT AGA ACA GGA GC GGT GAC AGC TAT CCA GTT ACT GTT | Â |
| Â | Inner | Exon 13F Exon 35R | CTC GCT CAC TCA CAT GGT AGT AGT G GCC CAA CAC CAT TTT CAA AGA CTC | 3406 bp |
4 | Outer | Exon:13F Exon:50R | GCT TCA AGA AGA TCT AGA ACA GGA GC CCA GTA GTG CTC AGT CCA GGG | Â |
| Â | Inner | Exon 13F Exon 50R | CTC GCT CAC TCA CAT GGT AGT AGT G GGT TTA CAG CCT CCC ACT CAG | 5720 bp |