Table 2 Antisense oligonucleotides used to induce the targeted skipping of murine dystrophin exons 19–25. The optimised cocktail consisting of nine individual AOs (No. 1–9) was used in all experiments to induce exon 19–25 removal. Skipping of single exons resulted in either in-frame (IF) or out-of-frame (OF) transcripts.

1

M19A(+35+65)

GCC UGA GCU GAU CUG CUG GCA UCU UGC AGU U

31

52

12

OF

2

M20A(+23+47)

GUU CAG UUG UUC UGA AGC UUG UCU G

25

44

5

OF

3

M20A(+140+164)

AGU AGU UGU CAU CUG UUC CAA UUG U

25

36

OF

4

M21D(+04-16)

AAG UGU UUU UAC UUA CUU GU

20

25

1

OF

5

M22D(+08-12)

AUG UCC ACA GAC CUG UAA UU

20

40

1

OF

6

M23D(+07-18)

GGC CAA ACC UCG GCU UAC CUG AAA U

25

52

1

IF

7

M24A(+16+40)

CAA CUU CAG CCA UCC AUU UCU GUA A

25

40

6

IF

8

M24A(+78+102)

GAG CUG UUU UUU CAG GAU UUC AGC A

25

40

IF

9

M25D(+06-14)

UAA ACU AGU CAU ACC UGG CG

20

45

1

IF

10

M23D(+02-18) *

GGC CAA ACC UCG GCU UAC CU

20

60

IF