Figure 6
From: Characterization of the ribonuclease activity on the skin surface
Trans-protection of mRNA against skin surface ribonuclease activity. Ribonuclease activity assay (negative images). Capped lacZ mRNA in the absence (w/o) or presence of the ribonuclease inhibitors RNasin® (R-in, final concentration 1 U/μl) or SUPERase· In™ (S-in, 1 U/μl) was incubated with ribonucleases of Homo sapiens hand surface (final dilution 4×) or of Mus musculus ear surface (20×) or with 2, 5 pg/μl ribonuclease A from Bos taurus pancreas at 37°C for increasing time (indicated by the wedge): Samples were taken immediately after addition of ribonucleases as well as 15 min and 60 min after. Samples without ribonucleases are indicated by a dash and were not incubated at 37°C.