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Figure 3 | Genetic Vaccines and Therapy

Figure 3

From: Differential intracellular distribution of DNA complexed with polyethylenimine (PEI) and PEI-polyarginine PTD influences exogenous gene expression within live COS-7 cells

Figure 3

Effect of microtubule disrupting agent, nocodazole, on microtubule morphology and PEI polyplex trafficking. Untreated (A) and nocodazole treated cells (D) displaying normal and disrupted microtubules, highlighted by anti-β-tubulin visualized using FITC secondary antibody. Nucleus stained with DAPI (B, E) and merged immunofluorescence displayed as (C, F). Distribution of PEI/pDNA-YOYO in untreated (G) and nocodazole treated (J) cells with MitoTracker staining (H, K). Merge MitoTracker and PEI/pDNA-YOYO in untreated (I) and nocodazole treated (L) cells. Scale bar = 10 μm. Images represent typical result of two individual slides viewed for each sample, analyzed 5 h post transfection.

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Genetic Vaccines and Therapy

ISSN: 1479-0556