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Figure 7 | Genetic Vaccines and Therapy

Figure 7

From: Streamlined design of a self-inactivating feline immunodeficiency virus vector for transducing ex vivodendritic cells and T lymphocytes

Figure 7

Transduction of primary murine DCs and T lymphocytes with LAW34/VSV-G and LAW34/RD114/TR. A) Flow cytometry analysis of DCs transduced with LAW34/VSV-G (thick line) or LAW34/RD114/TR (grey area) using PB protocol. Dotted line, untransduced, PB-treated cells. B) Efficiency of DC transduction by LAW34/VSV-G and LAW34/RD114/TR using the 3 protocols indicated. Percent GFP positive cells evaluated by flow cytometry 2 days PT. C) Intensity of GFP expression in the LAW34/RD114/TR transduced DCs in panel B, using protocols DT (thick line), PB (grey area), and DT/PB (grey line). Dotted line, untransduced DCs. D) Specific markers in DCs transduced with LAW34/RD114/TR using protocol PB or mock transduced, as examined 2 days PT. E) Flow cytometry analysis of T lymphocytes derived from murine spleen cells by ConA/IL-2 stimulation and transduced with LAW34/VSV-G (thick line) or LAW34/RD114/TR (grey area) using DT protocol. Dotted line, untransduced T lymphocytes. F) Efficiency of T lymphocyte transduction by LAW34/VSV-G and LAW34/RD114/TR using the protocols indicated.

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Genetic Vaccines and Therapy

ISSN: 1479-0556