Figure 2

Fluorescent PLGA microspheres were captured by antigen presenting cells after intramuscular administration. To determine the leukocyte subsets involved in the uptake process, mice received a single intramuscular injection of 6-coumarin labeled microspheres containing DNA-Hsp65/TDM, vector/TDM or empty microspheres (without plasmid DNA and TDM). After 7 days, the draining lymph nodes cells were collected for FACS analysis (CD80, CD86, CD11b, CD11c and class II MHC). Control group was injected with saline. (A): Number of cells positive for cell surface molecules CD80, CD86 and MHC class II; *p < 0.05 versus control formulations (vector/DMT and empty). (B): MFI increase expressed as a percentage from pcDNA3-Hsp65/TDM and pcDNA3/TDM formulations, normalized to empty formulation. The values represented in the graphs are the mean ± SD of three experiments.